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pNRF2 quantification HCS sections were immunostained with primary anti-pNRF2 antibodies (Abcam, Cambridge, UK, phospho-Ser40, Rabbit monoclonal antibody, Cat# ab76026, RRID: AB_1524049, Dilution 1:200) and Anti-Rabbit Alexa Fluor 647 antibodies (Invitrogen/Fisher Scientific, Goat Polyclonal, Cat# A-21245, RRID: AB_2535813, Dilution: 1:500) as previously described
In this paper, we investigate the impact of reducing agents on protein synthesis in a reconstituted cell-free protein synthesis system ( PURE system , Product name: PURE frex )
L-ARGININE L-Arginine is the bodys largest source of nitrous oxide, which relaxes blood vessels and improves circulation
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Briefly, HHDPCs (1 10 5 cells per well) were seeded on 6-well culture plates
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